What does the sulfosalicylic acid test detect?

The sulfosalicylic acid test detects albumin, globulins and Bence-Jones proteins at low concentrations. The sulfosalicylic acid test is the differential screening urine protein test for monoclonal light chain diseases i.e. multiple myeloma or pancreatic transplants with anastomosis.

What is sulfosalicylic acid used for?

The sulfosalicylic acid test is useful for a quick diagnosis of proteinuria. In a hypertensive pregnant patient the finding of a turbidity level of 4+ or 5+ would confirm the diagnosis of preeclampsia in 98% of the cases.

How do you test for sulfosalicylic acid?

Procedure

1. Check the pH of a portion of urine, if it is alkaline or neutral, add 10% acetic acid solution, drop by drop, until it is just acidic (about pH 6).
2. If the urine is cloudy, filter or centrifuge the urine (5 minutes, 2000-3000 rpm).
3. Take 2ml clear urine in a test tube.

What is postural proteinuria?

Orthostatic (also referred to as postural) proteinuria is characterized by an elevated protein excretion while in the upright position and normal protein excretion in a supine or recumbent position. It is the most frequent cause of isolated proteinuria in children, especially adolescents.

What other tests may be performed to quantitate proteinuria?

Additional tests might include:

• Blood test to measure the levels of creatinine (chemical waste products).
• Blood test to estimate the glomerular filtration rate (GFR).
• Blood test to measure all proteins in the serum.
• Imaging tests like CT scans and ultrasounds.
• Urine protein electrophoresis.
• Immunofixation blood test.

Which test is used for the turbidimetric screening of urine protein?

Turbidimetric Methods: SSA, SSSS and TCA Methods The total proteins present in urine were measured by precipitating the proteins with precipitating reagents, 3 % sulphosalicylic acid (SSA), 3 % sulphosalicylic acid in 7 % sodium sulphate (SSSS) and 3 % trichloroacetic acid (TCA).

Which of the following tests is a specific measure of glomerular filtration?

A glomerular filtration rate (GFR) is a blood test that checks how well your kidneys are working. Your kidneys have tiny filters called glomeruli. These filters help remove waste and excess fluid from the blood. A GFR test estimates how much blood passes through these filters each minute.

Is orthostatic proteinuria normal?

Orthostatic proteinuria is the most common type in children, especially in adolescent males. It is a benign condition without clinical significance. Persistent proteinuria can be glomerular or tubulointerstitial in origin.

How do you know if you have orthostatic proteinuria?

A urine protein-to-creatinine ratio can also help with the diagnosis. A normal urine protein/creatinine ratio in a recumbent position (less than 0.2mg/mg creatinine) with a high ratio in samples collected in a standing position establishes the diagnosis.

What is the sulfosalicylic acid test for protein?

Sulfosalicylic Acid Test An alternative method for measuring urine protein by dipstick in the office in patients with questionable proteinuria is the sulfosalicylic acid precipitation of protein in urine. This technique provides a more quantitative estimate of all the proteins present, including both albumin and the low molecular weight proteins.

What is the sulfosalicylic acid test for turbidity?

The sulfosalicylic acid test requires centrifugation of the urine followed by addition of 2.5 ml of the supernatant to 7.5 ml of 3% sulfosalicylic acid. The degree of turbidity is quantified as follows: Both tests are subject to the caveat that a concentration of protein is being tested.

What is the differential diagnosis of proteinuria?

The differential diagnosis of proteinuria is shown in Table 192.1. The primary diagnostic procedure is concomitant testing of the urine with both sulfosalicylic acid and dipstick. If only the former is positive, albuminuria is excluded and the differential diagnosis is that of monoclonal light chain diseases.

Does SSA detect albumin in proteinuria?

SSA detects all proteins, not just albumin. Proteinuria involving non-albumin proteins as well as albumin is seen more in tubular or overflow proteinuria. Iodinated radiocontrast also will interfere with the accuracy of the SSA test.