What is TRC shRNA?

The RNAi Consortium, or TRC, is a public-private effort based at the Broad whose mission is to create a shRNA library as well to validate tools and methods that will enable the scientific community to use RNAi to determine the function of human and mouse genes.

What is an RNAi library?

RNAi libraries will facilitate the functional characterization of genes in these species, either through studying smaller subsets of candidates or on a genomic scale. The design of RNAi reagents is key to obtaining reliable phenotypic data in large-scale RNAi experiments.

How do I order shRNA?

Order shRNA Online in 5 Easy Steps

1. Login to our site. Log in or sign up (if you haven’t already registered).
2. Search your gene. Search your gene of interest.
3. Select shRNA.
4. Select from the list of products displayed according to the species and format for which you are looking.
5. Select the specific clones and add to cart.

What is shRNA knockdown?

shRNA Mediated Gene Silencing Short hairpin RNA (shRNA) mediated gene silencing is a widely used approach for stable gene knockdown; these short RNA sequences can be expressed via viral or non-viral DNA vectors that encode shRNA.

What is Crispr screening?

CRISPR screening is used to find a small number of important genes or genetic sequences within a massive number of genetic sequences such as the entire genome. CRISPR screening can identify genes that influence many physiological effects, including drug resistance and drug sensitivity.

How does an RNAi screen work?

RNAi screening relies on the availability of genome-wide or other large-scale RNAi reagent libraries, with one or more unique RNAi reagent directed against each target gene. The appropriate RNAi reagent library for cell-based screening depends upon the cell type, approach and method of reagent delivery7.

How does shRNA knockdown work?

When shRNA is delivered using lentiviral vectors, the sequence encoding the shRNA is integrated into the genome and the knockdown effect is passed on to daughter cells, allowing for continued gene silencing. RNAi can also be induced by synthetic siRNAs between 19-21 base pairs with 2 nucleotide overhangs.

Does shRNA target mRNA?

shRNA versus siRNA Typically, short RNA molecules are created that are complementary to endogenous mRNA and when introduced into cells, bind to the target mRNA. Binding of the short RNA molecule to the target mRNA functionally inactivates the target mRNA and sometimes leads to degradation of the target mRNA.

Why shRNA is better than siRNA?

Recent in vitro studies have shown that shRNA produces fewer off-target effects than siRNA. In one study shRNA and siRNA of the same core sequence directed towards TP53 were applied to HCT-116 colon carcinoma cells in concentrations necessary to achieve comparable levels of target knockdown.

What is the shRNA library?

The RNAi Consortium shRNA Library. The RNAi Consortium, or TRC, is a public-private effort based at the Broad whose mission is to create a shRNA library as well to validate tools and methods that will enable the scientific community to use RNAi to determine the function of human and mouse genes.

What are the reagents used in the TRC shRNA library?

The reagents are composed of short hairpin sequences carried in lentiviral vectors arrayed in 96-well plates. The TRC shRNA library is distributed as bacterial glycerol stocks, plasmid DNA or lentiviral particles by Sigma-Aldrich and as bacterial glycerol stocks by Open Biosystems.

What is a Hannon-Elledge shRNA library?

The Hannon-Elledge shRNA libraries were created by Drs Greg Hannon (CSHL) and Steve Elledge (Harvard). Designed with features aimed at increasing gene knockdown efficiencies and versatility of use in RNAi screens these libraries provide solutions for rapid and high throughput applications of RNAi.

Which shRNAs are guaranteed to reduce target mRNA levels by 70%?

**For SMARTvector, GIPZ, and TRIPZ lentiviral shRNAs, at least one out of three constructs is guaranteed to reduce target mRNA levels by 70% or more when used in combination with the appropriately matched non-targeting and positive controls. Positive and negative constitutive shRNA controls with choice of seven promoters and three reporter options.