How does phenotypic screening work?

Phenotypic screening is defined as an assay which measures a desired, endogenous effect in a biological system, with that effect being agnostic of a defined target. In comparison target-based compound screening is defined as an assay where the measured effect is directly dependent upon a known target or pathway.

What is phenotypic based drug discovery?

Phenotypic drug discovery (PDD) is a disease-first paradigm to identify new medicines (see definitions, Table 1.1). The emphasis is on assays and strategies that recapitulate disease phenotypes and are agnostic to the molecular mechanisms—how the drugs work.

What is targeted screening in drug discovery?

Target validation measures the biological response it has to the drug to be clinically functional, and it is measured by the drug’s ability to induce it appropriately. (Takenaka 2008). Fragment-based screening is a technology used in target discovery.

Why is high throughput screening necessary?

The main goal of the HTS technique is to accelerate drug discovery by screening large compound libraries at a rate that may exceed a few thousand compounds per day or per week. It is of vital importance, because parallel and combinatorial chemical synthesis generates a vast number of novel compounds.

What is image based profiling?

Image-based profiling is a maturing strategy by which the rich information present in biological images is reduced to a multidimensional profile, a collection of extracted image-based features.

What are the two major approaches to drug discovery?

The two major CADD-based approaches are structure-based drug design, where protein structures are required, and ligand-based drug design, where ligand and ligand activities can be used to design compounds interacting with the protein structure.

What is phenotypic selection?

Phenotypic selection occurs when individuals with certain characteristics produce more surviving offspring than individuals with other characteristics. Although selection is regarded as the chief engine of evolutionary change, scientists have only recently begun to measure its action in the wild.

What is target based approach?

The target-based approach is a rational approach for screening drug candidates targeting a biomolecule that causes diseases. These approaches are widely used for drug discovery.

What is target based drug development?

A target-based drug discovery program focusing on a genetic target will have the goal of developing a drug that selectively modulates the effects of the disease-associated gene or gene product without affecting other genes or molecular mechanisms in the organism.

What is phenotypic approach?

From Wikipedia, the free encyclopedia. Phenotypic screening is a type of screening used in biological research and drug discovery to identify substances such as small molecules, peptides, or RNAi that alter the phenotype of a cell or an organism in a desired manner.