What does D2-40 positive mean?

In the central nervous system tissue, vacuolated cells found in cerebellar hemangioblastoma were demonstrated to be strongly positive with D2-40 antibody, and D2-40 is a useful marker to distinguish hemangioblastoma from metastatic clear cell renal cell carcinoma.

What does D2-40 stain?

D2-40 is a commercial monoclonal antibody directed against the M2a antigen, which is associated with testicular and extratesticular seminomas and intratubular germ cell neoplasms [9]. Also, D2-40 is a marker for lymphatic endothelium and derived carcinomas [10].

What is Calretinin positive?

A calretinin stain tests positive in most cases of mesothelioma. Like many other biomarkers, calretinin is not useful for detecting sarcomatoid cells. Sarcomatoid mesothelioma is the rarest cell type and the most difficult to treat.

Is mesothelioma CK7 positive?

This study has shown that the immunocytochemical profile of testicular malignant mesotheliomas is similar to those arising in the pleura, with diffuse positivity for calretinin, EMA, thrombomodulin, and CK7, and negative for CK20 and CEA.

What does WT 1 positive mean?

WT1-positive tumors included tumor of the stomach, prostate, and biliary and urinary systems, and malignant melanomas. A majority of the positive cases showed diffuse or granular staining in the cytoplasm, whereas ovarian tumors and desmoplastic small round cell tumors frequently showed nuclear staining.

What is moc31?

MOC-31 is an antibody that recently was reported to be useful in distinguishing adenocarcinoma from mesothelioma in tissue specimens. In this study we examined the utility of this antibody in pleural effusions.

What does CK5 6 positive mean?

Cytokeratin 5/6 is a positive marker for malignant pleural mesothelioma, found in more than three-fourths of cases. It is also found in certain types of lung cancers and breast cancers.

What are calretinin neurons?

Calretinin is a calcium-binding protein related to calbindin-D28k; both are present in different though overlapping sets of neurons in brains of birds and mammals. We describe in detail the pattern of calretinin immunoreactivity in the rat brain.

Is CK7 positive good?

For CK7, staining in > 10% of tumor cells was considered a positive sample. For CK20, staining in > 25% of tumor cells was considered positive (Fig. 1). Concerning MMR status, tumors with any apparent nuclear staining with MSH2, MSH6, PMS2, and MLH1 were considered MMR-proficient.

What is a WT1 marker?

Aims: Wilms tumor protein 1 (WT1) expression is used in gynecological pathology as a diagnostic marker of serous differentiation, and is frequently co-expressed with ER-α. Early phase studies on WT1 vaccine in gynecological cancers are ongoing.

Is d2-40 immunoreactivity a useful immunohistochemical marker of mesothelioma?

Compared to other immunohistochemical markers of mesothelioma, D2-40 was as sensitive as calretinin and more sensitive than cytokeratin 5/6 and WT1. We conclude that D2-40 immunoreactivity is sensitive for cells of mesothelial origin, and may be useful in the differential diagnosis of epithelioid malignant mesothelioma vs adenocarcinoma.

What is the d2-40 M2A antigen?

D2-40, oncofetal M2A antigen D2-40 was initially considered to be directed against the M2A antigen, a 40 kDa O-linked sialoglycoprotein found on the cell surface of testicular gonocytes, germ cell tumours lymphatic endothelium and mesothelial cells.

Is podoplanin a marker for sarcomatoid mesothelioma?

Despite some discouraging older data, podoplanin also appears to be a sensitive marker for sarcomatoid mesothelioma, with staining in up to 90% of tumors (13,28,32,34,37,49,50), although careful examination is required, since D2-40 staining of lymphatic spaces may mimic tumor staining (4). Podoplanin is imperfectly specific for mesothelioma.

Which immunohistochemical markers are used in the workup of sarcomatoid mesothelioma?

Padgett DM, Cathro HP, Wick MR, et al. Podoplanin is a better immunohistochemical marker for sarcomatoid mesothelioma than calretinin. Am J Surg Pathol2008;32:123-7. 10.1097/PAS.0b013e31814faacf [PubMed] [CrossRef] [Google Scholar]