Is pUC19 ampicillin resistant?

(A) The bla gene in pUC19, which confers ampicillin resistance, was replaced with fabI and its promoter region (pFab). The pUC19 multiple cloning site (MCS) is retained, however HincII, HindIII and PstI are not unique in pFab.

Is pUC19 kanamycin resistant?

pHSG299 is a kanamycin-resistant pUC cloning vector which contains the same consensus multicloning site as pUC19 (though Hind III and Sma I sites are not available). Using this vector, it is possible to transfer a DNA fragment from pUC18 to pUC19 without the preparation of DNA fragments.

Why does pUC19 have the ori segment?

The ori site, or origin of replication, is derived from the plasmid pMB1. pUC19 is small but has a high copy number. The high copy number is a result of the lack of the rop gene and a single point mutation in the ori of pMB1. The lacZ gene codes for β-galactosidase.

What is pUC19 vector?

pUC19 is a commonly used cloning vector that conveys the Amp resistance. The molecule is a small double-stranded circle, 2686 base pairs in length, and has a high copy number.

What is pUC19 plasmid?

pUC19 is a commonly used plasmid cloning vector in E.coli. The molecule is a double-stranded circular DNA (2686 base pairs in length). The pUC19 plasmid confers ampicillin resistance and complement defects in β-galactosidase in appropriate host strains.

What is the role of pUC19 in ampicillin resistance?

The pUC19 plasmid confers ampicillin resistance and complement defects in β-galactosidase in appropriate host strains.

What is the shape of pUC19?

pUC19 is a commonly used cloning vector that conveys the Amp resistance. The molecule is a small double-stranded circle, 2686 base pairs in length, and has a high copy number. pUC19 carries a 54 base-pair multiple cloning site polylinker that contains unique sites for 13 different hexanucleotide-specific restriction endonucleases (1).

What is the origin of replication in pUC19?

The ori site, or origin of replication, is derived from the plasmid pMB1. pUC19 is small but has a high copy number. The high copy number is a result of the lack of the rop gene and a single point mutation in the ori of pMB1. The lacZ gene codes for β-galactosidase.