What makes up the Fab region?

The Fab fragments contain the antigen binding site (CDR) of the antibody. Each Fab fragment is approximately 50 kDa and made up of the VL, CL, VH and CH1 domains.

What is the function of Fab?

The primary function of the Fab arms is to recognize and bind antigen. As previously mentioned, each variable domain (VH & VL) contains three CDRs, surface loops that together form an antigen binding site (Fig. 1(D) and (E)).

What is role of hinge region of immunoglobulin?

The hinge region is a stretch of heavy chains between the Fab and Fc portions. Its unique structure and position provide segmental flexibility, which is essential for normal functioning of antibodies (e.g., for crosslinking two antigens or binding two antigenic determinants on the same antigen molecule).

What is hinge region in antibody?

The hinge region is a flexible amino acid stretch in the central part of the heavy chains of the IgG and IgA immunoglobulin classes, which links these 2 chains by disulfide bonds.

What is correct about Fab fragment?

So, the correct answer is option B, Produced by seperation of heavy chain and light chain.

What is hinge region of antibody?

What are IgG1 and IgG2?

IgG2 has a shorter hinge than IgG1, with 12 amino acid residues. The lower hinge region of IgG2 (actually encoded by the CH2 region) also has a one amino acid deletion (lacking one of the double Glycines found at position 235-6), resulting in IgG2 having the shortest hinge of all the IgG subclasses.

What is the constant region of an antibody?

The constant region determines the mechanism used to destroy antigen. Antibodies are divided into five major classes, IgM, IgG, Iga, IgD, and IgE, based on their constant region structure and immune function. The variable region is further subdivided into hypervariable (HV) and framework (FR) regions.

What is Fab antibody?

Reduced nonspecific binding from Fc interactions (many cells have receptors that bind the Fc region)

Ability to control Fc-binding to Protein A or Protein G in experiments involving immunoprecipitation and Western blotting

More efficient penetration of tissue sections,resulting in improved staining in immunohistochemistry (IHC)

What is the role of a variable region of an antibody molecule?

Variable regions distinguish the antibodies made by one clone of B cells from the antibodies made by other clones. Variable and constant region. Each polypeptide chain of an immunoglobulin molecule contains an amino-terminal part and a carboxy-terminal part. The amino terminal part is called the variable region (V region) whereas the carboxy-terminal part is called the constant region (C region).

Which portion of the antibody binds to the antigen?

B cells,which originate in the bone marrow

T-cells which originate in the thymus

NK cells are also part of the lymphoid lineage but are part of the innate immunity